Study was performed to know the effects of Panax ginseng C.A. Meyer hairy root due to 60Co Y-ray irradiation. We irradiated the hairy roots under the various 60Co Y-ray; 0.5~4 Krad. The growth of hairy roots is inhibited over 3 Krad treatment. The lateral roots are used as a cell line after removing the apical meristem of hairy roots irradiated below 2 Krad. We selected 206 hairy root cell lines having various different growth rates and forms, and incubated in the 1/2 Murashige & Skoog(MS) medium in the absence of hormone. We selected 10 out of 206 showing superior growth.
Among those, Y-GHR 70 and Y-GHR 94 showed higher growth; 34.5, 44.7%, respectively. We observed shapable, sizable characteristics according to the width of the primary roots, the process formation of the lateral roots, and the growth of lateral roots. The discriminable cell line showed that primary root is thinner, and has a vigorous growth. 8 out of 10 had much more contents than control in the aspect of the ginsenoside. Y-GHR 59 and Y-GHR 94 showed higher contents; 19, 16.9%, respectively. Therefore, we selected Y-GHR 70, Y-GHR 94 as a superior cell line in the aspect of ginsenoside contents, and growth among those irradiated by Y-ray. According to content of ginsenoside, Rb2 effective in anticancer has 7.6% of Y-GHR 59. Rc, also effective in anticancer showed 16.2% content increasement of Y-GHR 69. It is thought that those lines will be effective in manufacturing ginsenoside. Gene analysis (VNTRP) related to the mutation is in progress.
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